Research - Laboratory/Non-Laboratory, Staff/Administrative
Under the general supervision of a faculty investigator or laboratory supervisor is responsible for performing routine analyses and tests on animal or human fluids and/or tissue or cultured cell lines. Work requires basic use of scientific methods, procedures and techniques gained through previous training and experience. Uses basic understanding of theory behind experiments and applies basic analytical skills to implement assigned projects, and to identify and troubleshoot situations needing special attention.
Specific Duties & Responsibilities
Follows established procedures or protocols and utilizes aseptic technique to perform experiments. These may require basic tissue culture, small animal surgery, genotyping, and/or cell separation methods, and laboratory methods/techniques associated with protein, lipid or carbohydrate biochemistry or molecular biology such as: purifications; quantitative and qualitative analyses, and localizations; as well as other functional and enzymatic assays.
May also perform recombinant DNA library construction; screening, isolation and mapping clones; oligonucleotide hybridization; plasmid subcloning, and/or screening DNA libraries. Operates basic laboratory equipment such as centrifuge, thermocycler, pH meter, analytical balance, scintillation counter, spectrophotometer, light microscope, and/or utilizes laminar flow hood. May also utilize sophisticated instrumentation such as high pressure liquid chromatograph and/or fluorescence or luminescence spectrophotometer.
May perform specific analyses using techniques such as column chromatography, affinity chromatography for protein purification, and/or ELISA's. Operates lab computers for data entry. May utilize in vivo models in experiments. Harvests primary cell cultures from blood and/or tissue specimens.
Maintains primary cell lines through procedures which may include passaging, cryopreservation, transfection and/or cloning. Uses various software applications to assemble, manipulate and/or format data and/or test reports. Prepares solutions, reagents, and stains following standard laboratory formulas and procedures. Monitors inventory levels, orders materials and supplies in accordance with established policies and procedures, counts orders upon receipt.
Bachelor’s degree in biology, chemistry, or related field required.
Some related post-undergraduate work experience preferred.
Classified Title: Research Technologist Working Title: Research Technologist Role/Level/Range: ACRO40/E/02/CC Starting Salary Range: $14.14 - $19.44/hr; Commensurate with experience Employee group: Full Time Schedule: Monday-Friday, 8:30 am - 5:00 pm, Up to 40 hrs/ week Exempt Status: Non-Exempt Location: 04-MD:School of Medicine Campus Department name: 10003533-SOM Neuro Sur Neurosurgical Oncology Personnel area: School of Medicine
The successful candidate(s) for this position will be subject to a pre-employment background check.
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During the Influenza ("the flu") season, as a condition of employment, The Johns Hopkins Institutions require all employees who provide ongoing services to patients or work in patient care or clinical care areas to have an annual influenza vaccination or possess an approved medical or religious exception. Failure to meet this requirement may result in termination of employment.
The pre-employment physical for positions in clinical areas, laboratories, working with research subjects, or involving community contact requires documentation of immune status against Rubella (German measles), Rubeola (Measles), Mumps, Varicella (chickenpox), Hepatitis B and documentation of having received the Tdap (Tetanus, diphtheria, pertussis) vaccination. This may include documentation of having two (2) MMR vaccines; two (2) Varicella vaccines; or antibody status to these diseases from laboratory testing. Blood tests for immunities to these diseases are ordinarily included in the pre-employment physical exam except for those employees who provide results of blood tests or immunization documentation from their own health care providers. Any vaccinations required for these diseases will be given at no cost in our Occupational Health office.
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