Research - Laboratory/Non-Laboratory, Staff/Administrative
Under the general supervision of a faculty investigator or laboratory supervisor, the Research Specialist II will focus on performing both routine assays and experimental techniques in the study of viruses (HBV, HCV, SARS-CoV-2, etc.) and the immune system. He/she will be expected to organize and present data to the faculty investigator. Additional responsibilities will include contributing to general maintenance of the laboratory, equipment, and supplies.
The candidate will be responsible for independently managing multiple ongoing research projects simultaneously, analyzing data, generating figures to summarize the data, and presenting the data to a faculty investigator in a clear and concise way and in a timely manner. Given clear directives they are expected to have the ability to forward research endeavors quickly and efficiently will little supervision.
Follow established procedures and utilizes aseptic technique to perform experiments associated with virology and immunology of HBV, HCV, SARS-CoV-2, etc.
Perform various biological assays to explore mechanisms influencing viral outcome and infectivity: nucleic acid extractions and purifications, gel electrophoresis, real-time PCR, ELISA, cell culture and transfection, molecular cloning, western blotting, sequencing, droplet digital PCR, LCM (laser capture microdissection), etc.
Perform, troubleshoot, and improve protocols for the neutralizing antibody cell-based assays for HCV and HBV.
Willingness to work with mice. Experience managing mice colonies is preferred. Basic surgical techniques including but not limited to tail vein injection, saphenous vein blood collection, liver dissection, and cardiac puncture.
Rigorous documentation of experimental planning, experimental methods, and experimental results updated on a daily basis. Responsible for operation of lab computers for data entry. Maintain accurate data records and laboratory notebook.
Along with the faculty investigator or senior lab staff, works to develop and institute protocols for routine laboratory analyses. Maintains and improves training procedures and is responsible for training entry-level employees on relevant techniques.
Operate basic laboratory equipment such as centrifuge, pH meter, analytical balance, spectrophotometer, light microscope, plate washer and/or utilize biological safety cabinet. This person will utilize sophisticated instrumentation such as flow cytometer, spectrophotometer, luminometer, thermal cycler, light cycler, droplet generator, LCM, etc.
Maintains and makes necessary adjustments to equipment, e.g. calibrating, alignment. Prepares solutions, reagents, and stains following standard laboratory formulas and procedures.
Monitors inventory levels, orders materials and supplies in accordance with established policies and procedures, counts orders upon receipt.
Additional responsibilities related to the daily laboratory operations will be assigned as needed.
Bachelor’s degree in biology, or Chemistry in a related field required.
Two (2) years of laboratory experience performing relevant techniques required.
Master's degree, with related graduate research, may substitute for experience to the extent permitted by the JHU equivalency formula.
JHU Equivalency Formula: 18 graduate degree credits (semester hours) may substitute for one year of experience.For jobs where equivalency is permitted, up to two years of non-related college course work may be applied towards the total minimum education/experience required for the respective job.
Master’s Degree in Biology.
Special Knowledge, Skills, and Abilities:
Basic Microsoft Office experience.
Expertise in immunological, molecular, and cell culture techniques. Basic techniques in animal handling.
Classified title: Research Specialist II
Working title: Research Specialist II
Role/Level/Range: ACRP 37.5/E/03/MB
Starting Salary Range: $38,920.08 - $53,517.60 (commensurate with experience)
Employee group: Fulltime
Employee Status: Exempt
Schedule: Monday-Friday –9:30am-6:00pm 37.5hrs/Wk
Location: 855 N. Wolfe Street, Baltimore, MD
Department name: 10002815-SOM DOM Nephrology
Personnel area (School): SOM – School of Medicine
The successful candidate(s) for this position will be subject to a pre-employment background check.
If you are interested in applying for employment with The Johns Hopkins University and require special assistance or accommodation during any part of the pre-employment process, please contact the HR Business Services Office at email@example.com. For TTY users, call via Maryland Relay or dial 711.
The following additional provisions may apply depending on which campus you will work. Your recruiter will advise accordingly.
During the Influenza ("the flu") season, as a condition of employment, The Johns Hopkins Institutions require all employees who provide ongoing services to patients or work in patient care or clinical care areas to have an annual influenza vaccination or possess an approved medical or religious exception. Failure to meet this requirement may result in termination of employment.
The pre-employment physical for positions in clinical areas, laboratories, working with research subjects, or involving community contact requires documentation of immune status against Rubella (German measles), Rubeola (Measles), Mumps, Varicella (chickenpox), Hepatitis B and documentation of having received the Tdap (Tetanus, diphtheria, pertussis) vaccination. This may include documentation of having two (2) MMR vaccines; two (2) Varicella vaccines; or antibody status to these diseases from laboratory testing. Blood tests for immunities to these diseases are ordinarily included in the pre-employment physical exam except for those employees who provide results of blood tests or immunization documentation from their own health care providers. Any vaccinations required for these diseases will be given at no cost in our Occupational Health office.
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